A multi-channel chip enabled synchronized reciprocating-flow of fluid for rapid, simultaneous, multiplex detection of inflammatory markers

Abstract

Infections are common in daily life and can lead to various acute diseases with complex symptoms. Rapid and accurate detection of multiple inflammatory markers using multi-channel microfluidic chips provides important guidance for controlling the development of infection. However, current fluid control methods for multi-channel microfluidic chips typically involve controlling the liquid in each channel separately and successively, which increases the complexity of control operations and the detection time. Here, we proposed a circularly-aligned parallel channels chip (CAPC chip) with multiple channels, and used this chip for the rapid, simultaneous detection of four inflammatory markers. We implemented the integrated fluid control method, and achieved the synchronized reciprocating-flow of fluid and the precise control of the flow velocity of fluid in multiple channels using a single air-source pressure control device. Then, we used the CAPC chip to perform rapid, simultaneous enzyme-linked immunosorbent assay (ELISA) detection of the 4 inflammatory markers (CRP, PCT, IL-6, SAA). The results showed that the CAPC chip can complete qualitative and quantitative detection of these 4 inflammatory markers within 5 min, which is the fastest ELISA detection for simultaneous detection of multiple protein biomarkers so far. Furthermore, we used the CAPC chip to detect these four inflammatory markers in simulated serum samples, and provide prompt information regarding the classification and severity of infection. These findings demonstrate the potential of the CAPC chip for rapid, simultaneous, multiplex immunoassays.