Non-linearity correction for variable signal analysis in mass spectrometry using discrete ion counters

Abstract

The response of a discrete ion counter is not perfectly linear due to count loss caused by dead time and pulse pileup. As a result, the output rate of the counter does not scale linearly with the input rate of ions reaching the detector. The value of a stable input rate can be determined from the measured output rate by inverting the throughput formula of the ion counter. However, when the input rate varies during the measurement, a mismatch between the average input rate and the average output rate becomes apparent. The resulting bias can be particularly significant when measuring transient signals. A correction procedure is proposed to calculate a better estimate of the average input rate from the observed mean and variance of the output rate. Implementation of this refined throughput formula is recommended to improve accuracy of mass spectrometry utilising discrete ion counters.