Ferulic acid identification in brown rice using untargeted metabolomics and network analysis and its effects in mitigating AFB1-induced hepatic damage

Abstract

Regular consumption of brown rice has been linked to reduce the probability of oxidative stress-related diseases, including diabetes, alzheimer's disease and cancer. The higher abundance of bioactive metabolites, including polyphenols and vitamins, in brown rice relative to white rice may contribute to its associated health benefits. Aflatoxin B1 (AFB1) as a kind of mycotoxin widely contaminated with grains is recognized as a primary risk factor for hepatocellular carcinoma based on its immense oxidative injury to humans. Therefore, the differential polyphenol metabolites between brown rice and white rice, as natural plant antioxidants, may also be able to mitigate the oxidative damage caused by AFB1. In this work, untargeted metabolomics approach was performed to identify the differential polyphenol metabolites between brown rice and white rice using ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). The result showed that 35 differential metabolites were found and classified into phenolic acids, flavonoids, lignans and coumarins. Among of them, ferulic acid (FA) was characterized a kind of differential metabolite which could mitigate AFB1-induced oxidative damage based on the interaction network and cell experiments. Furthermore, results of the mice experiment confirmed that FA mitigated AFB1-induced hepatic injury through a potential mechanism associated with the enhancement of antioxidant capacity, restoration of mitochondrial function and attenuation of AFB1-induced apoptosis. In conclusion, FA, identified between brown rice and white rice, could effectively alleviate AFB1-induced hepatic injury in vitro and in vivo. This study provides an important insight into the application of brown rice (abundant in FA) as a functional food.