Screening of the effect of new food prototypes based on fermented lentil and quinoa flours on the colonic microbiota of older adults through a static in vitro colonic fermentation model

Abstract

In view of the increasing demand for plant-based protein alternatives, along with the increased protein requirements of older adults, the formulation of new food concepts based on protein-rich ingredients from legumes or pseudo-cereals seems a promising approach. Previous studies have found that solid-state fermentation can improve the nutritional value and digestibility of plant-based commodities; however, scarce evidence exists regarding the effect on prebiotic potential. This study aimed to compare the effect of fermented and unfermented quinoa and lentil flours on the colonic microbiota, as well as that of new food prototypes (gels and breads) made with the flours. After simulating static colonic fermentation of the substrates by using a pool faecal inoculum obtained from four older adults, the microbiota composition (16S rRNA gene sequencing) and short-chain fatty acids (SCFA, gas chromatography GC-FID) were determined. The results showed statistically significant changes in the microbiota at the genus taxonomic level in the range of −5.8% to +17.6% relative abundance. Common findings in all experiments were increased Bacteroides, Acidaminococcus and Parabacteroides and decreased Asteroleplasma, Oscillospiraceae UCG-002 group and Alistipes, as well as increased SCFA production. Overall, the food matrix (flour, gel or bread) was the variable that most affected the changes in the microbiota composition and diversity, while the impact of fermentation of the lentils or quinoa was not statistically significant for most cases. Concretely, the gel-like prototypes produced the most beneficial changes in microbiota composition, while the bread-like formulations were more favourable for increased SCFA and decreased bSCFA production. In conclusion, new food prototypes based on lentil and quinoa flour could have beneficial prebiotic potential. However, few additional advantages were observed from previous solid-state fermentation for obtaining the fermented lentils and quinoa flour.