LivePyxel: Accelerating image annotations with a Python-integrated webcam live streaming

Abstract

The lack of flexible annotation tools has hindered the deployment of AI models in some scientific areas. Most existing image annotation software requires users to upload a precollected dataset, which limits support for on-demand pipelines and introduces unnecessary steps to acquire images. This constraint is particularly problematic in laboratory environments, where real-time data acquisition from instruments such as microscopes is increasingly common. In this work, we introduce \texttt{LivePixel}, a Python-based graphical user interface that integrates with imaging systems, such as webcams, microscopes, and others, to enable real-time image annotation. LivePyxel is designed to be easy to use through a simple interface that allows users to precisely delimit areas for annotation using tools commonly found in commercial graphics editing software. Of particular interest is the availability of Bézier splines and binary masks, and the software's capacity to work with non-destructive layers that enable high-performance editing. LivePyxel also integrates a wide compatibility across video devices, and it's optimized for object detection operations via the use of OpenCV in combination with high-performance libraries designed to handle matrix and linear algebra operations via Numpy effectively. LivePyxel facilitates seamless data collection and labeling, accelerating the development of AI models in experimental workflows. LivePyxel is freely available at https://github.com/UGarCil/LivePyxel

Supplementary files

Article information

Article type
Paper
Submitted
18 Sep 2025
Accepted
11 Jan 2026
First published
13 Jan 2026
This article is Open Access
Creative Commons BY-NC license

Digital Discovery, 2025, Accepted Manuscript

LivePyxel: Accelerating image annotations with a Python-integrated webcam live streaming

U. Garcilazo-Cruz, J. O. Okeme and R. Vargas-Hernandez, Digital Discovery, 2025, Accepted Manuscript , DOI: 10.1039/D5DD00421G

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