Rapid-scan electron paramagnetic resonance spectroscopy of nitroxide based spin systems

Abstract

Rapid-scan (RS) electron paramagnetic resonance (EPR) spectroscopy combined with site-directed spin-labeling (SDSL) is a powerful tool for investigating fast kinetics on biologically relevant timescales. This article provides an overview of the technique, highlighting the utility of nitroxide-based spin-labels and probes. Recent technical and methodological progress demonstrating the applicability of RS EPR is illustrated through examples in the field of chemical biology.