From the journal:

Chemical Communications

A label-free orthogonal dual-channel CRISPR-Cas platform for simultaneous detection of Mycobacterium tuberculosis and respiratory syncytial virus

Yanan Lin,a   Dawei Jiang,a   Xingpeng Dong,a   Yilin Li,a   Xiyue Wu,a   Ruoxuan Li,a   Feng Li,b   Dan Sun ORCID logo *a  and  Yanyan Yu ORCID logo *a  

* Corresponding authors

a School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China
E-mail: dsun1203@ntu.edu.cn, yuyanyan@ntu.edu.cn

b Laboratory Medicine Center, The Sixth People's Hospital of Nantong (Affiliated Nantong Hospital of Shanghai University), Nantong 226011, China

Abstract

A label-free, orthogonal dual-channel CRISPR-Cas platform is developed for the simultaneous detection of diverse pathogens. Cas12a is reported by a G4-PPIX complex, and Cas13 by a split Broccoli-DFHBI aptamer, which ensure exclusive channel specificity and eliminate optical cross-talk. Clinical tests on 50 samples show 100% concordance with PCR.

Graphical abstract: A label-free orthogonal dual-channel CRISPR-Cas platform for simultaneous detection of Mycobacterium tuberculosis and respiratory syncytial virus

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Article information

DOI
https://doi.org/10.1039/D5CC05269F
Article type
Communication
Submitted
13 Sep 2025
Accepted
10 Oct 2025
First published
21 Oct 2025

Chem. Commun., 2025, Advance Article

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A label-free orthogonal dual-channel CRISPR-Cas platform for simultaneous detection of Mycobacterium tuberculosis and respiratory syncytial virus

Y. Lin, D. Jiang, X. Dong, Y. Li, X. Wu, R. Li, F. Li, D. Sun and Y. Yu, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC05269F

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