We developed a label-free, self-propagating amplification strategy for ultrasensitive Terminal deoxynucleotidyl transferase (TdT) detection. The system integrates TdT-triggered poly-T extension, poly-A–primed synthesis, nicking-mediated recycling, and SYBR Green I–based fluorescence output, achieving robust signal amplification and assay performance.
B. Chen, H. Yang, Z. Sui, Y. Yao, L. Guo and J. Xu, Chem. Commun., 2025, Accepted Manuscript , DOI: 10.1039/D5CC04298D
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