Distinguishing isomeric N-methylhistidine peptidoforms by ion mobility mass spectrometry

Abstract

Histidine residues in proteins undergo histidine methyltransferase-catalysed Nτ-His and Nπ-His methylation. Here, we demonstrate that ion mobility spectrometry enables separation and identification of peptides possessing the isomeric Nτ-methyl-His and Nπ-methyl-His residues, leading to the assignment of the naturally occurring methyl-His isomer.

Graphical abstract: Distinguishing isomeric N-methylhistidine peptidoforms by ion mobility mass spectrometry

Supplementary files

Article information

Article type
Communication
Submitted
01 Jul 2025
Accepted
23 Jul 2025
First published
23 Jul 2025

Chem. Commun., 2025, Advance Article

Distinguishing isomeric N-methylhistidine peptidoforms by ion mobility mass spectrometry

N. Bilgin, F. Berthias, T. Ravnsborg, S. Ahmad, P. Emmel, J. Z. Kaminska, J. Drozak, O. N. Jensen and J. Mecinović, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC03712C

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