Temperature-dependence of membrane protein–lipid interactions in membranes

Abstract

Membrane protein–lipid interactions, like other biomolecular interactions, are often temperature-sensitive. Here, we use variable-temperature electrospray ionization mass spectrometry to investigate the temperature dependence of protein–lipids interactions from membranes. The findings reveal that specific lipid binding, and in some cases metal ion binding, to membrane proteins is significantly enhanced at elevated temperatures.