Issue 31, 2025

A strong H-bond between a cysteine and the catalytic center of a [NiFe]-hydrogenase

Abstract

Infrared spectroscopy at cryogenic temperatures was used to monitor protonation changes on an H+-accepting, nickel-coordinating active site cysteine of the H2/H+-cycling membrane-bound [NiFe]-hydrogenase from Cupriavidus necator. Surprisingly, we identified another cysteine in the outer coordination sphere forming a strong H-bond with a cysteine thiolate coordinating both nickel and iron of the catalytic center.

Graphical abstract: A strong H-bond between a cysteine and the catalytic center of a [NiFe]-hydrogenase

Supplementary files

Article information

Article type
Communication
Submitted
05 Feb 2025
Accepted
14 Mar 2025
First published
14 Mar 2025
This article is Open Access
Creative Commons BY license

Chem. Commun., 2025,61, 5778-5781

A strong H-bond between a cysteine and the catalytic center of a [NiFe]-hydrogenase

C. Karafoulidi-Retsou, S. Katz, S. Frielingsdorf, O. Lenz, I. Zebger and G. Caserta, Chem. Commun., 2025, 61, 5778 DOI: 10.1039/D5CC00646E

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