Sequential oxidation of l-lysine by a non-heme iron hydroxylase

Abstract

2-Oxoglutarate-dependent non-heme iron hydroxylases offer a direct route to functionalizing C(sp³)–H bonds across a range of substrates, making them prime candidates for chemoenzymatic synthetic strategies. Here, we demonstrate the ability of a non-heme iron L-lysine dioxygenase to perform sequential oxidation and computationally explore structural elements that promote this reactivity.