Issue 34, 2025
From the journal:

Chemical Communications

Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products†

Hui-Juan Cheng,a   Yue Liu,a   Hua-Dong Li,ac   Bin-Cheng Yin, ORCID logo ab   Pei-Qiang Ma ORCID logo *a  and  Bang-Ce Ye ORCID logo ab  

* Corresponding authors

a Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative In-novation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237, China
E-mail: pqma@ecust.edu.cn

b Institute of Engineering Biology and Health, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China

c National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, China

Abstract

We combined catalytic hairpin assembly (CHA) with the Cas12a system for detecting melamine adulteration. This system involved two-step signal conversion and two-level amplification, boosting the sensor's versatility and sensitivity. The sensor showed excellent specificity and applicability for melamine detection in dairy products, and was broadened to viral nucleic acid detection.

Graphical abstract: Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products

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Article information

DOI
https://doi.org/10.1039/D5CC00114E
Article type
Communication
Submitted
08 Jan 2025
Accepted
25 Mar 2025
First published
26 Mar 2025

Chem. Commun., 2025,61, 6300-6303

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Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products

H. Cheng, Y. Liu, H. Li, B. Yin, P. Ma and B. Ye, Chem. Commun., 2025, 61, 6300 DOI: 10.1039/D5CC00114E

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