Renming Liu, Jun Qiu, Yifen Huang, Ziyi Wang and Peng Zheng
Chem. Commun., 2025,61, 4355-4358
DOI:
10.1039/D4CC06617K,
Communication
Open Access Learn more..An intracellular protein ligation system using a truncated variant of OaAEP1 (OaAEP1 core) to ligate and polymerize Ig domains in E. coli is developed, enabling real-time polyprotein synthesis without enzyme activation and external ligation. This approach yields functional, mechanically stable polyproteins and expands AEP applications in synthetic biology and biomaterials.
