Ion transport and membrane channel formation using a peptidomimetic in droplet interface bilayers

Abstract

Herein, we present a synthetic peptidomimetic, TBP2, which forms artificial membrane channels within droplet interface bilayers (DIBs). Real-time electrophysiology via TIRF microscopy, and single-channel recordings, we demonstrate the ability of TBP2 to mediate high-conductance transport of Na⁺ and K⁺ across the DIBs, highlighting its potential for nanobiotechnology applications in cell-free systems.