Nanophotonic platform for the highly sensitive detection of trypsin enzyme in human urine

Abstract

Trypsin is an important indicator of pancreatic disease. We introduce a nanophotonic test for the detection of trypsin. Our approach is based on the hydrolysis of β-casein by trypsin. We demonstrate 0.1 ng mL⁻¹ of trypsin detection in human urine and across the clinically relevant range.