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RSC Chemical Biology

Novel terbium-sensitizing peptide substrates for cyclin-dependent kinase 5 (CDK5) and their demonstration in luminescence kinase assays

Jason L. Heier, ORCID logo a   Dylan J. Boselli ORCID logo a  and  Laurie L. Parker ORCID logo *a  

* Corresponding authors

a Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, USA
E-mail: llparker@umn.edu

Abstract

Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. A substrate designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.

Graphical abstract: Novel terbium-sensitizing peptide substrates for cyclin-dependent kinase 5 (CDK5) and their demonstration in luminescence kinase assays

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Article information

DOI
https://doi.org/10.1039/D5CB00189G
Article type
Communication
Submitted
21 Jul 2025
Accepted
05 Sep 2025
First published
11 Sep 2025
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2025, Advance Article

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Novel terbium-sensitizing peptide substrates for cyclin-dependent kinase 5 (CDK5) and their demonstration in luminescence kinase assays

J. L. Heier, D. J. Boselli and L. L. Parker, RSC Chem. Biol., 2025, Advance Article , DOI: 10.1039/D5CB00189G

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