Issue 25, 2025

A simple, sensitive, and green method for the determination of 5-fluorouracil exploiting luminol electrochemiluminescence

Abstract

This study presents a simple, sensitive, and environmentally friendly analytical method based on luminol electrochemiluminescence (ECL) for the quantification of 5-fluorouracil. The approach relies on the suppression of the luminol/hydrogen peroxide ECL signal in the presence of the analyte using a screen-printed carbon electrode (SPE). The total solution volume containing luminol, hydrogen peroxide, and the drug was only 40 μL, ensuring significant reagent savings and minimal waste generation, which qualifies it as a green analytical method. Under optimized conditions, the ECL method displayed a wide linear response range from 0.3 to 10 000 nmol L−1. The proposed technique achieved a detection limit of 0.1 nmol L−1 and was successfully applied for the determination of 5-fluorouracil in artificial urine and plasma samples, with recovery values ranging from 95.00% to 100.18% and 97.54% to 103.74%, respectively.

Graphical abstract: A simple, sensitive, and green method for the determination of 5-fluorouracil exploiting luminol electrochemiluminescence

Article information

Article type
Paper
Submitted
13 May 2025
Accepted
20 May 2025
First published
12 Jun 2025

Anal. Methods, 2025,17, 5210-5220

A simple, sensitive, and green method for the determination of 5-fluorouracil exploiting luminol electrochemiluminescence

B. R. Demétrio, D. E. P. Souto, S. M. Silva, F. S. Damos and R. D. C. S. Luz, Anal. Methods, 2025, 17, 5210 DOI: 10.1039/D5AY00825E

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