Development of an effective H2S-activatable fluorescent probe for imaging in drug-induced living cells†

Abstract

Hydrogen sulfide (H2S) is a toxic gas signaling molecule which plays multiple physiological regulatory roles in both physiological and pathological processes. A novel fluorescent probe T1 is designed and synthesized for detecting H2S in biological systems. Based on the ICT mechanism, thiolysis of the 2,4-dinitrophenyl ether which is used as the fluorescence quenching group occurs after the addition of H2S, releasing the original fluorescent group and accompanied by the recovery of the fluorescence signal (emission peak at 615 nm) with the detection limit of 0.42 μM. Probe T1 displays low cytotoxicity and excellent ability to recognize exogenous and endogenous H2S in living cells. It could recognize H2S produced by LPS induced cell inflammation, monitor the release of H2S by the H2S prodrug ADT-OH in cells, and serve as an anti-counterfeiting dye for printing technology, confirming the reliability of the probe. Therefore, probe T1 can be used as a fast and effective tool for detecting H2S in vitro and in vivo.

Article information

Article type
Paper
Submitted
06 May 2025
Accepted
20 Jul 2025
First published
29 Jul 2025

Anal. Methods, 2025, Accepted Manuscript

Development of an effective H2S-activatable fluorescent probe for imaging in drug-induced living cells†

Y. HOU, J. Li, Y. Ding, J. Zhuoma, Y. Zhao, Z. Langjie and M. Yang, Anal. Methods, 2025, Accepted Manuscript , DOI: 10.1039/D5AY00763A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements