Nitrogen-Doped Carbon Dots for Ultrasensitive Detection of Hg2+ in Traditional Chinese Medicine and Bioimaging Applications

Abstract

A nitrogen-doped carbon dots (N-CDs)-based fluorescent sensor was developed for ultrasensitive and selective detection of mercury ions (Hg2+) in complex matrices, such as Panax notoginseng (Burk.) F. H. Chen (Sanqi). The synthesized N-CDs exhibited intense blue fluorescence (quantum yield: 35%) with an ultra-small size of 1.93±0.53 nm. Leveraging dynamic quenching mechanisms, the sensor achieved a linear detection range of 0-50 μM for Hg2+ and an exceptionally low limit of detection (LOD) of 0.2825 nM, outperforming existing CD-based sensors. The N-CDs demonstrated remarkable selectivity for Hg2+ in the presence of interfering ions and complex herbal components. Hg2+ in Sanqi samples was quantified by fluorescence analysis (25.5–26.8 μg/kg, RSD<2.0%) and cross-validated via Inductively Coupled Plasma Mass Spectrometry (ICP-MS) (24.8–26.2 μg/kg, RSD<1.0%), demonstrating N-CDs’ reliability for precise Hg2+ detection. Additionally, the N-CDs exhibited excellent biocompatibility and were successfully applied for cellular imaging in A549 cells, showcasing their dual functionality in environmental monitoring and biomedical diagnostics.