Freeze-Drying Enhances ToF-SIMS Imaging of Biological Tissues via Structural and Metabolic Preservation

Abstract

In the ToF-SIMS in situ analysis of mouse liver tissue, freeze-drying is superior to room-temperature drying and chemical fixation, minimizing lipid spillage while maintaining structural integrity. Freeze-drying preserves tissue structure with minimal lipid loss, whereas room-temperature drying leads to lipid spreading and chemical fixation results in background noise. Freeze-drying balances structural integrity, molecular preservation, and spectral clarity, making it the optimal ToF-SIMS prep method. Although the method was tested on lipid-rich liver tissue, it provides a framework for tissue-specific preparation of ToF-SIMS.