A near-infrared photoelectrochemical aptasensor based on Yb-Bi2S3/AuNPs nanocomposite for determining miRNA-155 a biomarker for allergic rhinitis

Abstract

MiRNA-155 is a reliable biomarker for cancers and allergic rhinitis, also plays a critical role in regulating gene expression and mediating the allergic TH2 process. The accurate determination of miRNA-155 will be significant for diagnosing allergic rhinitis, especially for children. Herein, Yb-Bi2S3 nanorods were prepared through a hydrothermal method. Gold nanoparticles (AuNPs) were assembled onto Yb-Bi2S3 nanorods surface to provide a Yb-Bi2S3/AuNPs nanocomposite. Yb-Bi2S3/AuNPs nanocomposite was used as a photoactive element for fabricating a near-infrared photoelectrochemical aptasensing platform by assembling the aptamer for specific recognition of miRNA-155. The photocurrent variation of the photoelectrochemical aptasensor before and after recognizing miRNA-155 is linearly related to the miRNA-155 concentration in the range of 1.0 fmo l L-1 ~ 1.0 nmo l L-1. The detection limit was calculated to be 0.34 fmol L-1. The developed photoelectrochemical aptasensor was successfully employed for determining miRNA-155 in serum samples with satisfactory results, indicating possessing practical applications in the determination of miRNA-155 in real samples.