Issue 11, 2025

A microfluidic DNA sensor array for real-time screening of early-stage lung cancer by simultaneous detection of multiple miRNAs

Abstract

We present a DNA sensor array for simultaneous detection of four targeted miRNAs in serum for early-stage lung cancer screening. Owing to effective microfluidic enrichment coupled with ultrasensitive solid–liquid capacitive sensing, the detection limit is as low as 12.84–24.69 aM, with a dynamic range of 0.1 fM to 10 pM. The response time for each miRNA detection is only 30 s, and the total detection time is 120 s. The selectivity reaches above 10 000 : 1. The miRNA concentrations in the patients with early-stage lung cancer are determined to be 6.5–263 times higher than those in healthy individuals, demonstrating the excellent feasibility of this sensor array for clinical applications for real-time screening of early-stage lung cancer.

Graphical abstract: A microfluidic DNA sensor array for real-time screening of early-stage lung cancer by simultaneous detection of multiple miRNAs

Supplementary files

Article information

Article type
Communication
Submitted
21 Jan 2025
Accepted
25 Feb 2025
First published
26 Feb 2025

Anal. Methods, 2025,17, 2376-2379

A microfluidic DNA sensor array for real-time screening of early-stage lung cancer by simultaneous detection of multiple miRNAs

J. Zhang, W. Yao, X. Mao, X. Hu, L. Lv and H. Qi, Anal. Methods, 2025, 17, 2376 DOI: 10.1039/D5AY00109A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements