Detection of blood glucose based on Aggregation induced luminescence effect in 4-formyl-3-hydroxybenzoic acid@ZIF-8 and Förster resonance energy transfer ratio fluorescence method between Aggregation-induced emission molecules and diallyl phthalate

Abstract

A novel and simple ratio fluorescence detection method for H2O2 and glucose was proposed on the basis of the Aggregation-induced luminescence effect of the Aggregation-induced emission (AIE) molecule 4-formyl-3-hydroxybenzoic acid (FHBA) in ZIF-8 and the mechanism of Fluorescence resonance energy transfer (FRET) between the complex FHBA@ZIF-8 and Diallyl phthalate (DAP). First, glucose was oxidized to H2O2 under the catalysis of glucose oxidase. The latter further oxidized o-phenylenediamine in the presence of horseradish peroxidase to generate DAP yellow fluorescent compound (560 nm). The fluorescence of FHBA@ZIF-8 at 445 nm can be effectively quenched because the ultraviolet absorption spectrum of DAP overlaps well with the emission spectrum of FHBA@ZIF-8. Therefore, a highly sensitive ratiometric fluorescence detection method for detecting H2O2 and glucose was constructed using the change of the ratio of fluorescence intensity at two wavelengths (F560/F445). The linear range of H2O2 detection by this method is 1–150 μM, and the detection limit is 0.21 μM. The linear range of glucose detection is 1–100 μM, and the detection limit is 0.17 μM. The method was successfully applied to the detection of glucose in human serum samples, and it has broad potential in clinical diagnosis and research