Issue 15, 2025

Electroanalysis of meat discoloration: beef sarcoplasm extract with storage time

Abstract

Electrochemical techniques offer a promising approach for monitoring redox-active biomolecules involved in meat discoloration. Building on our previous identification of characteristic redox peaks in beef extracts associated with myoglobin oxidation (S. B. Kasthuri Dias, S. Bhandari, S. A. Devage, J. A. Avery, R. Kumar, R. Ramanathan and S. Krishnan, J. Am Chem. Soc., 2024, 146, 30728–30732, DOI: 10.1021/jacs.4c09375), we present an extended investigation into electrochemical changes over longer meat storage times. Square wave voltammetry revealed frequency-dependent peak potential shifts over a period of 9 days, indicative of a quasi-reversible electron transfer process. Spiking experiments with purified myoglobin in the meat extract confirmed its predominant contribution to the observed voltammetric signals. After 9 days of storage, peak currents at −0.26 V (redox-active myoglobin heme center) and +0.38 V (overall oxidation of meat components) increased by 68.9% and 270.3%, respectively, compared to relative changes of 37.3% and 61.7% in a shorter-duration study. These electrochemical findings showed a strong correlation with spectrophotometrically determined metmyoglobin accumulation (r = 0.98, P < 0.001). The results provide insight into redox transformations during meat spoilage and contribute to the development of electrochemical tools for evaluating meat quality.

Graphical abstract: Electroanalysis of meat discoloration: beef sarcoplasm extract with storage time

Article information

Article type
Paper
Submitted
14 Mar 2025
Accepted
15 Jun 2025
First published
17 Jun 2025
This article is Open Access
Creative Commons BY license

Analyst, 2025,150, 3289-3297

Electroanalysis of meat discoloration: beef sarcoplasm extract with storage time

S. Bhandari, S. A. Devage, R. Kumar, R. Ramanathan and S. Krishnan, Analyst, 2025, 150, 3289 DOI: 10.1039/D5AN00301F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements