Issue 11, 2025

A GQD/g-C3N4-modified nanochip for electrochemical detection of dengue serotype 3

Abstract

A graphene quantum dot/2D graphitic carbon nitride (GQD)/g-C3N4-based ultrasensitive DNA biosensor was constructed using 5′ aminated DNA probes immobilized onto the surface of a screen-printed paper electrode (SPPE) for detecting DENV 3 (dengue serotype 3). FTIR, UV-Vis, TEM, and particle size measurements were used to characterize the synthesized nanocomposites. These results were then used to fabricate an affordable DNA sensor based on an SPPE that detects DENV 3. Initially, bioinformatics methods were used to develop DNA probes unique to DENV 3. The findings indicated that the most effective DNA probe for identifying DENV 3 was a 20-nucleotide sequence, which was immobilized onto a GQD/g-C3N4-modified SPPE. The electrochemical response was studied via differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The electrode surface at each step of fabrication was characterized via FE-SEM, EIS and FTIR. The developed DNA sensor showed excellent analytical performance in terms of LOD and sensitivity. In CV, the LOD was 1 pg μl−1, with a sensitivity of 7993 ng μA−1 mm−2. However, in DPV, the LOD and sensitivity were 1 pg μl−1 and 11 116 ng μA−1 mm−2, respectively. This method should be preferable in the future for detecting DENV 3 in biological and clinical samples.

Graphical abstract: A GQD/g-C3N4-modified nanochip for electrochemical detection of dengue serotype 3

Supplementary files

Article information

Article type
Paper
Submitted
09 Jan 2025
Accepted
21 Apr 2025
First published
22 Apr 2025

Analyst, 2025,150, 2430-2439

A GQD/g-C3N4-modified nanochip for electrochemical detection of dengue serotype 3

R. Poria, A. Kaushal, S. Verma, S. K. Ramasamy, G. Ghodake, D. Kumar and S. Gupta, Analyst, 2025, 150, 2430 DOI: 10.1039/D5AN00035A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements