Issue 8, 2024

Towards predictive control of reversible nanoparticle assembly with solid-binding proteins

Abstract

Although a broad range of ligand-functionalized nanoparticles and physico-chemical triggers have been exploited to create stimuli-responsive colloidal systems, little attention has been paid to the reversible assembly of unmodified nanoparticles with non-covalently bound proteins. Previously, we reported that a derivative of green fluorescent protein engineered with oppositely located silica-binding peptides mediates the repeated assembly and disassembly of 10-nm silica nanoparticles when pH is toggled between 7.5 and 8.5. We captured the subtle interplay between interparticle electrostatic repulsion and their protein-mediated short-range attraction with a multiscale model energetically benchmarked to collective system behavior captured by scattering experiments. Here, we show that both solution conditions (pH and ionic strength) and protein engineering (sequence and position of engineered silica-binding peptides) provide pathways for reversible control over growth and fragmentation, leading to clusters ranging in size from 25 nm protein-coated particles to micrometer-size aggregate. We further find that the higher electrolyte environment associated with successive cycles of base addition eventually eliminates reversibility. Our model accurately predicts these multiple length scales phenomena. The underpinning concepts provide design principles for the dynamic control of other protein- and particle-based nanocomposites.

Graphical abstract: Towards predictive control of reversible nanoparticle assembly with solid-binding proteins

Supplementary files

Article information

Article type
Paper
Submitted
22 Jan 2024
Accepted
28 Jan 2024
First published
30 Jan 2024

Soft Matter, 2024,20, 1935-1942

Towards predictive control of reversible nanoparticle assembly with solid-binding proteins

Y. Cai, X. Qi, J. Boese, Y. Zhao, B. Hellner, J. Chun, C. J. Mundy and F. Baneyx, Soft Matter, 2024, 20, 1935 DOI: 10.1039/D4SM00094C

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