Volume 3, 2024

Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging

Abstract

Herein, a chromone-based simple reversible fluorescent “turn-on” probe, HMCP [6-(hydroxymethyl)-N′-((6-methyl-4-oxo-4H-chromen-3-yl)methylene)picolinohydrazide], was successfully utilized to detect Al3+ over a group of other coexisting metal cations in MeOH/H2O (9 : 1, v/v) (HEPES buffer, pH = 7.2). The “turn on” emission response along with the effective enhancement of the fluorescence intensity upon addition of Al3+ can be attributed to the inhibition of photo-induced electron transfer (PET) and C[double bond, length as m-dash]N isomerization, as well as the initiation of chelation-enhanced-fluorescence (CHEF). The HMCP sensor binds Al3+ in a 1 : 1 stoichiometry with an excellent binding constant and good detection limit on the orders of 103 M−1 and 10−7 M, respectively. The mode of binding interaction between HMCP with Al3+ was evidenced by 1H NMR titration, HRMS, and Job's plot analyses. Theoretical calculations and molecular logic gate applications were also used to demonstrate the binding mode. A DNA binding study was also executed to elucidate the possible bioactivity of the probe and found that HMCP interacts with DNA more effectively than the other analogues studied. Furthermore, the applicability of the probe in a live cell imaging study indicated that HMCP is highly efficient for the detection of exogenous Al3+ in living cells. In addition, real water sample analysis and a dip-stick experiment demonstrate that the probe can be used in a wide range of practical and convenient applications.

Graphical abstract: Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
30 Jun 2024
Accepted
10 Sep 2024
First published
18 Sep 2024
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2024,3, 1866-1876

Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging

A. Maji, D. Mitra, A. Biswas, M. Ghosh, R. Naskar, S. Gharami Nabendu Murmu and T. K. Mondal, Sens. Diagn., 2024, 3, 1866 DOI: 10.1039/D4SD00242C

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