Volume 3, 2024

An imidazo[1,2-a]pyridine-functionalized xanthene fluorescent probe for naked-eye detection of Hg2+ and its application in cell imaging and test strips

Abstract

An imidazo[1,2-a]pyridine-functionalized xanthene dye (Rh-Ip) was designed and developed as a fluorescent probe (Rh-Ip-Hy) for Hg2+ by introducing spirolactam to the molecule. The probe Rh-Ip-Hy with a ring-closed spirolactam structure reacts with Hg2+ to form a fluorescent ring-opened spirolactone structure. Due to its asymmetric structure and the presence of a Lewis base site, the probe exhibits a larger Stokes shift and higher pH tolerance than the traditional xanthene dyes and probes. Furthermore, the probe is highly selective to Hg2+ within a wide pH range of 5.0–11.0. The probe Rh-Ip-Hy also exhibits low cytotoxicity and can be used for the detection of Hg2+ in living HeLa cells through fluorescence imaging. Finally, a paper-based test strip was prepared and successfully applied for the detection of Hg2+ in tap water and lake water samples.

Graphical abstract: An imidazo[1,2-a]pyridine-functionalized xanthene fluorescent probe for naked-eye detection of Hg2+ and its application in cell imaging and test strips

Supplementary files

Article information

Article type
Paper
Submitted
23 Mar 2024
Accepted
27 Apr 2024
First published
05 May 2024
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2024,3, 1062-1067

An imidazo[1,2-a]pyridine-functionalized xanthene fluorescent probe for naked-eye detection of Hg2+ and its application in cell imaging and test strips

X. Han, P. Zhao, M. Tang, L. Yang, Q. Wang and S. Zhang, Sens. Diagn., 2024, 3, 1062 DOI: 10.1039/D4SD00090K

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