Multiplexed aptasensor for detection of acute myocardial infraction (AMI) biomarkers

Abstract

Acute myocardial infarction (AMI) is a leading global cause of death. Diagnosis is challenging as cardiac biomarkers are only detectable for a few hours after AMI onset, and current methods are time-consuming and lack selectivity. Therefore, multiple immunological test systems have great importance for rapid and accurate diagnosis. In this context, we developed a rapid immunodiagnostic sensor platform for simultaneous electrochemical detection of cardiac troponin T (cTnT), troponin I (cTnI), and C-reactive protein (CRP) using nanostructured gold-modified laser-scribed graphene (LSG). Aptamer sensors were integrated into the LSG platform for selective AMI biomarkers sensing. Clinical validation was performed on biomarkers from blood samples of 51 AMI patients and 9 healthy controls. Limits of detection were 1.65 ng mL⁻¹ cTnT, 2.58 ng mL⁻¹ cTnI, and 1.84 ng mL⁻¹ CRP. The analytical results determined by the developed platform were compared with the routine standard values of the same patients to prove the accuracy of aptasensors. Sensor results agreed well with standard laboratory assays, highlighting the accuracy of the test platform. The cTnT, cTnI and CRP multiplexed sensor platform demonstrates excellent performance for rapid and sensitive AMI screening.