Issue 22, 2024

High-throughput single biomarker identification using droplet nanopore

Abstract

Biomarkers are present in various metabolism processes, demanding precise and meticulous analysis at the single-molecule level for accurate clinical diagnosis. Given the need for high sensitivity, biological nanopore have been applied for single biomarker sensing. However, the detection of low-volume biomarkers poses challenges due to their low concentrations in dilute buffer solutions, as well as difficulty in parallel detection. Here, a droplet nanopore technique is developed for low-volume and high-throughput single biomarker detection at the sub-microliter scale, which shows a 2000-fold volume reduction compared to conventional setups. To prove the concept, this nanopore sensing platform not only enables multichannel recording but also significantly lowers the detection limit for various types of biomarkers such as angiotensin II, to 42 pg. This advancement enables direct biomarker detection at the picogram level. Such a leap forward in detection capability positions this nanopore sensing platform as a promising candidate for point-of-care testing of biomarker at single-molecule level, while substantially minimizing the need for sample dilution.

Graphical abstract: High-throughput single biomarker identification using droplet nanopore

Supplementary files

Article information

Article type
Edge Article
Submitted
18 Dec 2023
Accepted
28 Mar 2024
First published
10 Apr 2024
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2024,15, 8355-8362

High-throughput single biomarker identification using droplet nanopore

L. Zhang, C. Zhong, T. Huang, L. Zhang, F. Yan and Y. Ying, Chem. Sci., 2024, 15, 8355 DOI: 10.1039/D3SC06795E

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