Issue 28, 2024, Issue in Progress

Two rhenium compounds with benzimidazole ligands: synthesis and DNA interactions

Abstract

Two rhenium compounds: cis-tetrachlorotetrabenzimidazoldirhenium(III) chloride – I and tetrabenzimidazoldioxorhenium(V) – II have been synthesized and characterized. X-ray data are presented for the new complex II. I and II show strong emission that has been used to investigate their interaction with several non-canonical DNA structures. Both compounds have a quenching effect on the fluorescence intensity upon addition of the investigated oligonucleotides; I was more selective for binding G4-than II. Association constant values obtained for I and II with G-quadruplexes reached 106 M−1, which suggests a strong interaction between both complexes and these sequences. FRET-melting assays show that I and II have a rather high level of stabilization of ckit1 and ckit2 quadruplexes. I is toxic against macrophages RAW267.7 only in high concentrations, while complex II shows no toxicity against these cells. I and II accumulate inside cells in different degrees. Molecular dynamic simulation studies have provided insights into the binding modes of II with ckit1 and ckit2 G-quadruplexes. The results obtained show the DNA binding activity of the rhenium complexes and their ability to be players in the anti-cancer fight since they can bind to non-canonical DNA forms in oncogene promoters, accumulate in some cancer cells, and influence the cancer cells microenvironment.

Graphical abstract: Two rhenium compounds with benzimidazole ligands: synthesis and DNA interactions

Supplementary files

Transparent peer review

To support increased transparency, we offer authors the option to publish the peer review history alongside their article.

View this article’s peer review history

Article information

Article type
Paper
Submitted
09 Apr 2024
Accepted
03 Jun 2024
First published
20 Jun 2024
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2024,14, 19787-19793

Two rhenium compounds with benzimidazole ligands: synthesis and DNA interactions

N. Shtemenko, C. Galiana-Rosello, A. Gil-Martínez, S. Blasco, J. Gonzalez-García, H. Velichko, O. Holichenko, O. Shtemenko and E. García-España, RSC Adv., 2024, 14, 19787 DOI: 10.1039/D4RA02669A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements