Issue 3, 2024

Differences in interaction of graphene/graphene oxide with bacterial and mammalian cell membranes

Abstract

Graphene, a single layer, hexagonally packed two-dimensional carbon sheet is an attractive candidate for diverse applications including antibacterial potential and drug delivery. One of the knowledge gaps in biomedical application of graphene is the interaction of these materials with the cells. To address this, we investigated the interaction between graphene materials (graphene and graphene oxide) and plasma membranes of cells (bacterial and mammalian cells). The interactions of four of the most abundant phospholipids in bacteria and mammalian plasma membranes with graphene materials were studied using density functional theory (DFT) at the atomic level. The calculations showed that the mammalian phospholipids have stronger bonding to each other compared to bacterial phospholipids. When the graphene/graphene oxide sheet is approaching the phospholipid pairs, the bacterial pairs exhibit less repulsive interactions, thereby a more stable system with the sheets was found. We also assembled bacterial and mammalian phospholipids into liposomes. We further observed that the bacterial liposomes and cells let the graphene flakes penetrate the membrane. The differential scanning calorimetry measurements of liposomes revealed that the bacterial liposomes have the lowest heat capacity; this strengthens the theoretical predictions of weaker interaction between the bacterial phospholipids compared to the mammalian phospholipids. We further demonstrated that graphene oxide could be internalized into the mammalian liposomes without disrupting the membrane integrity. The results suggest that the weak bonding among bacteria phospholipids and less repulsive force when graphene materials approach, result in graphene materials interacting differently with the bacteria compared to mammalian cells.

Graphical abstract: Differences in interaction of graphene/graphene oxide with bacterial and mammalian cell membranes

Supplementary files

Article information

Article type
Paper
Submitted
23 Oct 2023
Accepted
14 Dec 2023
First published
15 Dec 2023
This article is Open Access
Creative Commons BY license

Nanoscale, 2024,16, 1156-1166

Differences in interaction of graphene/graphene oxide with bacterial and mammalian cell membranes

V. Lanai, Y. Chen, E. Naumovska, S. Pandit, E. Schröder, I. Mijakovic and S. Rahimi, Nanoscale, 2024, 16, 1156 DOI: 10.1039/D3NR05354G

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