Glucose-doped calcium carbonate nanospheres: a new signal-transduction tag for nano-labelled immunosorbent assay (NLISA)

Abstract

An innovative signal-transduction tag with glucose-doped calcium carbonate nanospheres was utilized as the label of a secondary antibody to design a nano-labelled immunosorbent assay (NLISA) of a carcinoembryonic antigen. The signal was achieved on a personal glucometer via the released glucose molecules from the nanospheres under acidic conditions after formation of the sandwiched immune complexes. The enzyme and the spectrometer in the conventional enzyme-linked immunosorbent assay (ELISA) were replaced by a glucose-doped calcium carbonate nanosphere and a glucometer.