Issue 1, 2024

New buffer systems for photopainting of single biomolecules


We present newly developed buffer systems that significantly improve the efficiency of a photochemically induced surface modification at the single molecule level. Buffers with paramagnetic cations and radical oxygen promoting species facilitate laser-assisted protein adsorption by photobleaching (LAPAP) of single fluorescently labelled oligonucleotides or biotin onto multi-photon-lithography-structured 2D and 3D acrylate scaffolds. Single molecule fluorescence microscopy has been used to quantify photopainting efficiency. We identify specific cation interaction sites for members of the cyanine, coumarin and rhodamine classes of fluorophores using quantum mechanical calculations. We show that our buffer systems provide an up to three-fold LAPAP-efficiency increase for the cyanine fluorophore, while keeping excitation parameters constant.

Graphical abstract: New buffer systems for photopainting of single biomolecules

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Article information

Article type
04 Aug 2023
03 Oct 2023
First published
07 Oct 2023
This article is Open Access
Creative Commons BY license

RSC Appl. Interfaces, 2024,1, 110-121

New buffer systems for photopainting of single biomolecules

C. Naderer, H. Krobath, D. Sivun, G. Gvindzhiliia, T. A. Klar and J. Jacak, RSC Appl. Interfaces, 2024, 1, 110 DOI: 10.1039/D3LF00125C

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