Lipase-entrapped colloidosomes with light-responsive wettability for efficient and recyclable Pickering interfacial biocatalysis

Abstract

Light-responsive Pickering interfacial biocatalysis system (LPIB) is desirable because of its convenient catalyst recovery, product separation and clean external stimuli trigger. However, the recyclability of LPIB is still limited due to enzyme inactivation. Herein, lipase-entrapped colloidosomes as particulate emulsifiers and biocatalysts are fabricated by one step co-assembly of lipases and TiO₂ nanoparticles (with light-responsive wettability). The obtained LPIB exhibits a 4.31-fold enhancement of enzyme activity compared with the traditional biphasic system. Impressively, the LPIB maintains nearly 90% of initial enzyme activity even after 20 recycles, which is the highest among the currently reported LPIBs. It is attributed that TiO₂ nanoparticles layer of colloidosome surface reflect UV light and protect enzymes from photodegradation. This green platform can be widely applied to construct recyclable and efficient biphasic biocatalytic systems.