Aflatoxin B1 exposure causes splenic pyroptosis by disturbing the gut microbiota-immune axis

Abstract

Aflatoxin B₁ (AFB₁) causes serious immunotoxicity and has attracted considerable attention owing to its high sensitivity and common chemical–viral interactions in living organisms. However, the sensitivity of different species to AFB₁ widely varies, which cannot be explained by the different metabolism in species. The gut microbiota plays a crucial role in the immune system, but the interaction of the microbiota with AFB₁-induced immunotoxicity still needs to be determined. Our results indicated that AFB₁ exposure disrupted the structure of the gut microbiota and damaged the gut barrier, which caused translocation of microbiota metabolites, lipopolysaccharides, to the spleen. Subsequently, pyroptosis of the spleen was activated. Interestingly, AFB₁ exposure had little effect on the splenic pyroptosis of pseudo-germfree mice (antibiotic mixtures eliminated their gut microbiota, ABX). Then, fecal microbiota transplant (FMT) and sterile fecal filtrate (SFF) were employed to validate the function of the gut microbiota and its metabolites in AFB₁-induced splenic pyroptosis. The AFB₁-disrupted microbiota and its metabolites significantly promoted splenic pyroptosis, which was worse than that in control mice. Overall, AFB₁-induced splenic pyroptosis is associated with the gut microbiota and its metabolites, which was further demonstrated by FMT and SFF. The mechanism of AFB₁-induced splenic pyroptosis was explored for the first time, which paves a new way for preventing and treating the immunotoxicity from mycotoxins by regulating the gut microbiota.