Multi-omics revealed the mechanisms of AgNP-priming enhanced rice salinity tolerance

Abstract

Rice is highly susceptible to salt stress. Increasing the salt tolerance of rice is critical to reduce yield loss. Herein, we investigated the possibility of using an AgNP-based priming method (seed soaking (SP) and leaf spraying (LP)) to enhance rice salt tolerance. Under saline conditions, both SP (40 mg L−1) and LP (∼0.15 mg per plant) significantly increased the biomass (10.4–13.4%) and height (6.6–6.9%) of 6-week-old rice seedlings. In addition, SP significantly increased chlorophyll a (7.3%) and carotenoid (7.9%) content as well as total antioxidant capacity (10.5%), whereas it decreased malondialdehyde (MDA) content (16.9%) in rice leaves. These findings indicate that AgNP priming, especially SP, improved the salt tolerance of rice seedlings. A life cycle field study conducted in a real saline land revealed that SP significantly increased the rice grain yield by 25.8% compared to hydropriming. Multi-omics analyses demonstrated that AgNP priming induced metabolic and transcriptional reprogramming in both seeds and leaves. Notably, both SP and LP upregulated osmoprotectants in seeds and leaves. Furthermore, several transcriptional factors (TFs), such as WRKY and NAC, and salt-tolerance related genes, including the high-affinity K+ channel gene (OsHKT2;4, OsHAK5), the Ca2+/proton exchanger (CAX4), and the cation/Ca2+ exchanger (CCX4), were upregulated in leaves. Omics data provide a deep insight into the molecular mechanisms for enhanced salinity tolerance. Together, the results of this study suggest that seed priming with AgNPs can enhance the salt tolerance of rice and increase rice yield in saline soil, which provides an efficient and simple way to engineering salt-tolerant rice.

Graphical abstract: Multi-omics revealed the mechanisms of AgNP-priming enhanced rice salinity tolerance

Supplementary files

Article information

Article type
Paper
Submitted
28 Jul 2024
Accepted
29 Sep 2024
First published
25 Oct 2024

Environ. Sci.: Nano, 2024, Advance Article

Multi-omics revealed the mechanisms of AgNP-priming enhanced rice salinity tolerance

S. Chen, Z. Pan, J. R. Peralta-Videa and L. Zhao, Environ. Sci.: Nano, 2024, Advance Article , DOI: 10.1039/D4EN00685B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements