Mobility of Soil-Biodegradable Nanoplastics in Unsaturated Porous Media Affected by Protein-Corona

Abstract

Soil-biodegradable plastic has been increasingly used as mulches in agriculture, which provides not only agronomical benefits but also in-situ disposal and biodegradation options. However, soil-biodegradable plastic mulches inevitably fragment into micro- and nanoplastics during biodegradation, which can reside in soils or migrate into deep soils, where they may not degrade readily due to reduced microbial activity. To date, little is known about the transport of soil-biodegradable micro- and nanoplastics in soils. Here, we studied the transport of soil-biodegradable nanoplastics (∼200 nm) made of polybutylene adipate co-terephthalate (PBAT) in unsaturated sand (proxy for soil). Specifically, we studied the mobility of pristine and weathered PBAT nanoplastics in the absence and presence of proteins (positively charged lysozyme and negatively charged bovine serum albumin, pH = 7.7). We found that (1) both pristine and the weathered PBAT nanoplastics were mobile; (2) positively charged lysozyme formed protein-coronas around PBAT nanoplastics and inhibited the transport; and (3) decreased water saturation promoted the retention of PBAT nanoplastics via physical straining. These results suggest that soil-biodegradable nanoplastics fragmented from soil- biodegradable plastic mulches are mobile and may readily migrate into deep soil layers, but positively charged proteins and unsaturated flow would prevent such transport via formation of protein-corona and physical straining.