Selective interactions of Co2+–Ca2+–concanavalin A with high mannose N-glycans

Abstract

Concanavalin A (ConA) has an intrinsic binding affinity to carbohydrates. Here, we obtained Co²⁺–Ca²⁺–ConA (2.83 Å, PDB: 8I7Q) via X-ray crystallography by substituting native ConA (Mn²⁺–Ca²⁺); it has binding selectivity for high-mannose N-glycan similar to native ConA. Our findings may thus inform antiviral reagent design.