Ternary recognition fluorescent probe for lysosome acidification counter-ion studies via Cl−, K+, and pH

Abstract

Lysosomal acidity relies on H⁺ inflow, which requires counter-ion flows (Cl⁻ and K⁺) to balance charge. A lysosome targeting ternary recognition fluorescent probe for Cl⁻, K⁺, and pH was developed for lysosome acidification counter-ion research. The probe was used to study counter-ion changes when the Cl⁻ channel was blocked and under oxidative pressure.