Fully negatively-charged (FNC) layer-by-layer nanofilms were successfully assembled on a living cell surface for the first time using only poly(acrylic acid) (PAA) by introducing strain-promoted click chemistry to crosslink PAA layers. The resulting nanofilms retained their negative charges and showed higher adsorption of positively-charged molecules without affecting the cell viability.
Z. Zhang, J. Zeng and M. Matsusaki, Chem. Commun., 2024, 60, 7781 DOI: 10.1039/D4CC02782E
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