Issue 35, 2024

The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore detection

Abstract

Through controlling the ssDNA product length of rolling circle amplification with AcyNTP, here we develop a nanopore signal enhancement strategy (STSS), which can successfully transfer the short oligonucleotide targets into long ssDNAs with appropriate lengths that can generate significant translocation currents. By labelling the RCA product with tags such as tetrahedral structures and isothermal amplicons, the resolution, signal specificity, and target range of the STSS can be further extended.

Graphical abstract: The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore detection

Supplementary files

Article information

Article type
Communication
Submitted
28 Feb 2024
Accepted
30 Mar 2024
First published
01 Apr 2024

Chem. Commun., 2024,60, 4723-4726

The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore detection

J. Yu, C. Yu, Y. Li, C. Yu, Y. Wang, R. Wu and B. Li, Chem. Commun., 2024, 60, 4723 DOI: 10.1039/D4CC00961D

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