Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease

Sheng Lei; Zi Liu; Qidong Zhang; Zezhi Li; Gaiping Li; Lina Zou; Guobi Chai; Jian Mao; Baoxian Ye; Jianxun Zhang

doi:10.1039/D3CC06058F

Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease†

Sheng Lei,^ae Zi Liu,^b Qidong Zhang,^a Zezhi Li,^af Gaiping Li,

^c Lina Zou,

^c Guobi Chai,^a Jian Mao,^ad Baoxian Ye

*^c and Jianxun Zhang*^a

Author affiliations

* Corresponding authors

^a Zhengzhou Tobacco Research Institute of China National Tobacco Company, Zhengzhou 450001, P. R. China
E-mail: jxzh258@163.com, yebx@zzu.edu.cn

^b Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, P. R. China

^c College of Chemistry, Zhengzhou University, Zhengzhou 450001, P. R. China

^d Beijing Life Science Academy, Beijing, P. R. China

^e College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, P. R. China

^f School of Light Industry, Beijing Technology and Business University, Beijing 100048, P. R. China

Abstract

A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was investigated for ultrasensitive detection of miRNA. Impressively, addition of duplex-specific nuclease (DSN) just once drove the twice-walk strategy, making the strategy simpler. With the advantages of being simple, rapid and ultrasensitive, the biosensor offers potential for use in early clinical diagnosis.

Chemical Communications

Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease†

Abstract

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Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease

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