Issue 6, 2024

Mining proteomes for zinc finger persulfidation

Abstract

Hydrogen sulfide (H2S) is an endogenous gasotransmitter that signals via persulfidation. There is evidence that the cysteine residues of certain zinc finger (ZF) proteins, a common type of cysteine rich protein, are modified to persulfides by H2S. To determine how frequently ZF persulfidation occurs in cells and identify the types of ZFs that are persulfidated, persulfide specific proteomics data were evaluated. 22 datasets from 16 studies were analyzed via a meta-analysis approach. Persulfidated ZFs were identified in a range of eukaryotic species, including Homo sapiens, Mus musculus, Rattus norvegicus, Arabidopsis thaliana, and Emiliania huxley (single-celled phytoplankton). The types of ZFs identified for each species encompassed all three common ZF ligand sets (4-cysteine, 3-cysteine-1-histidine, and 2-cysteine-2-hisitidine), indicating that persulfidation of ZFs is broad. Overlap analysis between different species identified several common ZFs. GO and KEGG analysis identified pathway enrichment for ubiquitin-dependent protein catabolic process and viral carcinogenesis. These collective findings support ZF persulfidation as a wide-ranging PTM that impacts all classes of ZFs.

Graphical abstract: Mining proteomes for zinc finger persulfidation

Supplementary files

Article information

Article type
Paper
Submitted
27 Jun 2023
Accepted
03 Dec 2023
First published
13 May 2024
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2024,5, 572-585

Mining proteomes for zinc finger persulfidation

H. Li, A. T. Stoltzfus and S. L. J. Michel, RSC Chem. Biol., 2024, 5, 572 DOI: 10.1039/D3CB00106G

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