Fabrication and properties of temperature-responsive imprinted sensors based on fluorescently labeled yeast cells via MVL ATRP

Abstract

Temperature-responsive yeast cell-imprinted sensors (CIPs/AuNPs/Ti₃C₂T_x/AuNPs/Au) were prepared based on fluorescein isothiocyanate labeled yeast cells (FITC-yeast) via metal-free visible-light-induced atom transfer radical polymerization (MVL ATRP). Here, N-isopropyl acrylamide (NIPAM) was used as a temperature-responsive functional monomer, α-methacrylic acid (MAA) was chosen as an auxiliary functional monomer, N,N′-methylene bisacrylamide (MBA) was used as a cross-linker, and FITC-yeast was selected as both a template and photocatalyst. Under the optimal conditions, the detection range of the yeast cell-imprinted sensor toward yeast cells was 1.0 × 10² to 1.0 × 10⁹ cells per mL, and the detection limit was 11 cells per mL (S/N = 3), with a linear equation of ΔI (μA) = 8.44 log[C (cells per mL)] + 7.62 (R² = 0.993). The sensor showed good selective recognition in the presence of interfering substances such as autolyzed yeast cells (AY), dead yeast cells (DY), human mammary epithelial cells (MCF-10A), human breast cancer cells (MCF-7) and Escherichia coli (EC). The sensor also had good consistency and reproducibility. Finally, spiked recovery experiments were performed to investigate the recognition of yeast cells in the actual sample using the yeast cell-imprinted sensor. The spiked recoveries were all in the range of 98.5–108.0%, and the RSD values were all less than 4%, indicating that the sensor had good application prospects.