Issue 30, 2024

A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(ii) complex with BSA and DNA complemented by docking studies

Abstract

A fluorescent molecule, pyridine-coupled bis-anthracene (PBA), has been developed for the selective fluorescence turn-on detection of Cu2+. Interestingly, the ligand PBA also exhibited a red-shifted ratiometric fluorescence response in the presence of water. Thus, a ratiometric water sensor has been utilized as a selective fluorescence turn-on sensor for Cu2+, achieving a 10-fold enhancement in the fluorescence and quantum yield at 446 nm, with a lower detection limit of 0.358 μM and a binding constant of 1.3 × 106 M−1. For practical applications, sensor PBA can be used to detect Cu2+ in various types of soils like clay soil, field soil and sand. The interaction of the PBA-Cu(II) complex with transport proteins like bovine serum albumin (BSA) and ct-DNA has been investigated through fluorescence titration experiments. Additionally, the structural optimization of PBA and the PBA-Cu(II) complex has been demonstrated by DFT, and the interaction of the PBA-Cu(II) complex with BSA and ct-DNA has been analyzed using theoretical docking studies.

Graphical abstract: A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(ii) complex with BSA and DNA complemented by docking studies

Supplementary files

Article information

Article type
Paper
Submitted
12 Apr 2024
Accepted
28 Jun 2024
First published
02 Jul 2024

Anal. Methods, 2024,16, 5263-5271

A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(II) complex with BSA and DNA complemented by docking studies

V. S, A. Nag and A. K. Das, Anal. Methods, 2024, 16, 5263 DOI: 10.1039/D4AY00675E

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