Issue 21, 2024

Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells

Abstract

Glyphosate is a widely used broad-spectrum herbicide in agriculture and horticulture to control a variety of weeds and undesirable plants. However, the excessive use of glyphosate has raised a number of environmental and human health concerns. It is urgent to develop tools to detect glyphosate. Herein, a novel dual-signal probe CCU–Cu2+ was designed and synthesized on the basis of CCU. CCU exhibited excellent selectivity and great sensitivity for Cu2+ which were based on both fluorescence “turn-off” reaction and comparative color visualisation methods. Due to the strong chelating ability of glyphosate on Cu2+, the CCU–Cu2+ complex was applied to glyphosate detection in practical samples. The experimental results in vitro showed that the CCU–Cu2+ complex was highly selective and rapid, with a low detection limit (1.6 μM), and could be recognised by the naked eye in the detection of glyphosate. Based on the excellent properties of the CCU–Cu2+ complex, we also constructed a smartphone-assisted detection sensing system for glyphosate detection, which has the advantages of precision, sensitivity, and high interference immunity. Moreover, the CCU–Cu2+ complex was also successfully employed for exogenous glyphosate imaging in living cells. These characteristics demonstrated that CCU–Cu2+ holds significant potential for detection and imaging of glyphosate in bio-systems.

Graphical abstract: Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells

Supplementary files

Article information

Article type
Paper
Submitted
29 Feb 2024
Accepted
26 Apr 2024
First published
01 May 2024

Anal. Methods, 2024,16, 3364-3371

Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells

X. Lin, T. Chen, J. Hu, X. Mao, M. Liu, R. Zeng, Q. Zhong and W. Chen, Anal. Methods, 2024, 16, 3364 DOI: 10.1039/D4AY00380B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements