Issue 8, 2024

A label-free and immobilization-free approach for constructing photoelectrochemical nucleic acid sensors utilizing DNA–silver nanoparticle affinity interactions

Abstract

Efficient and affordable nucleic acid detection methods play a pivotal role in various applications. Herein, we developed an immobilization-free and label-free strategy to construct a photoelectrochemical nucleic acid biosensing platform based on interactions between silver nanoparticles and DNA. First, CRISPR-Cas12a exhibited a trans-cleavage effect on adenine nucleotide sequences upon recognizing the target DNA. The resulting adenine nucleotide sequences of varying lengths then engaged in interactions with silver nanoparticles, leading to a solution characterized by distinct light transmittance. Subsequently, the solution was positioned between the light source and the photoelectrode, strategically impacting the photon absorption step within the photoelectrochemical process. Consequently, the detection of nucleic acid was accomplished through the analysis of the resultant photocurrent signal. The developed platform exhibits a detection limit of 0.06 nM (S/N = 3) with commendable selectivity. The innovative use of adenine nucleotide sequences as cost-effective probes interacting with silver nanoparticles eliminates the need for complex interfacial immobilization processes, significantly simplifying the fabrication of DNA sensors. The outcomes of our research present a promising pathway for advancing the development of economically feasible miniature DNA sensors.

Graphical abstract: A label-free and immobilization-free approach for constructing photoelectrochemical nucleic acid sensors utilizing DNA–silver nanoparticle affinity interactions

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
19 Jan 2024
Accepted
27 Feb 2024
First published
28 Feb 2024

Analyst, 2024,149, 2272-2280

A label-free and immobilization-free approach for constructing photoelectrochemical nucleic acid sensors utilizing DNA–silver nanoparticle affinity interactions

J. Yi, J. Dong, Y. Zheng, L. Liu, J. Zhu and H. Tang, Analyst, 2024, 149, 2272 DOI: 10.1039/D4AN00098F

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