Issue 28, 2023

Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo

Abstract

Charge-driven inclusion complex formation in live cells was examined using a degradation-prone fluorescent protein and a series of protein cages. The results show that sufficiently strong host–guest ionic interaction and an intact shell-like structure are crucial for the protective guest encapsulation.

Graphical abstract: Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo

Supplementary files

Article information

Article type
Communication
Submitted
06 Apr 2023
Accepted
22 Jun 2023
First published
10 Jul 2023
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2023,11, 6540-6546

Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo

D. Zakaszewski, L. Koziej, J. Pankowski, V. V. Malolan, N. Gämperli, J. G. Heddle, D. Hilvert and Y. Azuma, J. Mater. Chem. B, 2023, 11, 6540 DOI: 10.1039/D3TB00754E

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